SynthBioPoster

Synthetic Biology for Artists and Designers 2

10th May 2010

• Biology - individual understanding and definition.
• Brief History of Biology - Scientists/Microbiologists (Video)
? Anton Von Leeuwenhoek
? Gregor Mendel
? Robert Hooke
? Louis Pasteur
? John Baptiste Van Helmont
? Joseph Jackson Lister
? Theodore Schwann
? Rosalyn Franklin
? Watson & Crick
• Evolution – Brief understanding and controversies
• DNA, Protein – Introduction
• Bacteria E.Coli
• Readings – PDF
? Scientific America – Engineering Life
? Synthesizing DNA of microorganisms for various purposes
? Sequencing of DNA
? Cost factors involved
? Comparison to Moore’s Law
? Human Genome
• Video – (BBC)-The Cell-The Hidden Kingdom
Homework - Reading (PDF)
1. Life: What a concept!

11th May 2010

• Recap of yesterday’s learning
• Video lecture by David Sadava
? Expression of genes
? Phenotypes and Alleles
? The Nucleus
? Totipotent Cells
? Cloning
? Genes, chromosomes
? Human genetic information
? Cell processing
• Introduction to Synthetic Biology by Andrew Hessels
• Video – Darwin’s theory on evolution
Homework
1.
2.

12th May 2010

• Video Lecture by David Sadava
? Epidemiology
? Lung Cancer gene
? Mutations
? Requirements of a DNA
? Responsibilities of a Protein
? Variety in DNA
? Somatic and Sex Cells
? Virus
? DNA & its properties
? Structure of a DNA
? Replication of DNA
• Video of Watson and his story of the discovery of the DNA model at TED.com
• Readings – Applying engineering to biology
• Reading – Adventures in Synthetic Biology
Homework
1. Reading
2. Online Research

13th May 2010

1. Video Lecture by David Sadava
? Spider web theory of protein
? Protein production
? Structure of protein
? Synthesis of Protein
? Human protein synthesis
? Types of proteins
? Expression of Proteins
? Gene – Protein relationship
? Metabolism
? Thermodynamics
? Mechanism, Cell Theory and Evolution
? Ricin and its discovery
? RNA and its role
? Transcription and Translation
? Nirenberg’s Experiment
? Mutations and changes in base pairs
• Video tutorial on DNA replication
• Video tutorial on Protein synthesis
Homework
1. Exercise – Make a piece of art form using any LIVING form that should have the
capability to grow over a period of two months.

14th May 2010

1. Video Lecture by David Sadava
Genomes – further details
?
Mutations & Mutagens
?
Cancers caused by mutation of genes by radiation
?
Genetic Damage
?
Leukaemia
?
Scientist Renato del Bucco’s work
?
Hierarchical sequencing
?
Shotgun sequencing – Craig Ventor
?
Bioinformatics
?
Information from a genetic sequence
?
a. Coding sequence
b. Protein amino acid sequence
c. Gene control sequences
Sequential Genomics
?
First genome to be sequenced
?
Hypothetical suggestion of 24000 genes in a human being
?
Synthetic Biology - making artificial genes to bring about a desired result
?
Scientist Werner Arber
?
Restriction Endonuclease
?
How a bacteria prevents Viral infections
?
DNA Mapping
?
Recombinant DNA
?
Stanley Cohen & Herbert Boyer’s Experiment
?
Goals for making rDNA
?
Transgenic animals and plants
?
Naked and Vector DNA
?

17th May 2010-05-17

1. Video Lecture by David Sadava
? Isolation of genes
? PKU and dystrophy
? Methods of analyzing DNA
? Reverse Genetics
? Dystrophine Gene and Utrophine
? Gene Therapy
? Nucleic Acid Hybridization
? Genome Library
? DNA Microarray
? Metastasis
2. Exercise - Think of your own creature. Design it. Explain detailed mechanisms using
diagrams. This organism could be hypothetical. Name it.
3. Make a scientific paper on your creature
4. Demonstration of Gabriel’s current prototype of visually representing genetic sequenced
data of a human mitochondria.
Homework – develop and complete creature.

18th May 2010

1. Video Documentary – BBC – The Cell – Chemistry of Life
2. Video Documentary – BBC – The Cell – The Spark of Life
3. Discussion of Creatures, peer feedback and progress

19th May 2010

Center for Genomic Gastronomy @ Srishti, Bangalore, India from genomic gastronomy on Vimeo.

1. Presentation by Zac on The center for Genomic Gastronomy
? What is Biotechnolgy
? Perspective of food by all living things
? Geohackers, Biohackers, Geoengineers, Planetcrafters
? Agriculture Biodiversity
? Agrinformatics
? Metadata and food Genomes
? Food System Adaptation
? Agriculture Terrorism
? First world eating issues
? Cuisinformatics
2. Extraction of DNA

20th May 2010

1. Presentation of Abstracts of New Creatures
? The Booster Cell
? Supercule
? Thermo Cell
2. Exercise – Create another new creature which does not necessarily have to be a
microorganism or even possible to exist. Completely hypothetical, imaginary of fantastical is
great!

21st May 2010

Minutes of the Class
1. Discussion of ideation on creation of machines.

22nd May 2010

Minutes of the Class
1. Video Documentary by Craig Ventor
? Scientific Breakthrough – First fully Synthetic Cell to be cultutred.
? Genome copied from Mycoplasma Genatalium
? Genome synthetically prepared in the lab
? Introduced into yeast cells to complete transformation
? Introduced into host bacterial cell which transforms to new organism
? Watermarked with 46 authors, web address and mail ID
? Significance – vaccine production and Oil Spill reduction
2. Artist & other Research
? Stellarc
? Symbiotica
? Orlan

25th May 2010

1. Visit to NCBS
? Tour of the Campus
? Meeting with PhD student Mehra and Navneet
? Introduction to the lab space
? Discussion over Science Institutes and their pros and cons
? Tour of other labs with various workflow instruments

26th May 2010

Minutes of the Class
1. Video Tutorial by David Sadava
? Bioremediation – using organism to improve the environment
? Fluorescence in nature and different organisms
? TNT used as example
? Degraded by Psuedomonas Putida
? Integrated Pest Management
? Bacteria in decomposition
? Bacteria in Waste Water Treatment
? Extremophiles
? PCR and its significance
2. List of materials required to build the art form made from a living organism
3. Artist & other Research
? Institution of Applied Autonomy
? Center for Post Natural History
? George Gessert

27th May 2010

1. Video Documentary by Kary Mullis on the making of the PCR
2. Artist & other Research
? Adam Zaretsky
? Eduardo Kac
? Patricia Piccinni
3. Field work on material for art form construction.

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